Two patients present to the hospital with nausea, vomiting and diarrhea that started 12 hours after ingestion of a meal they prepared with foraged mushrooms. What qualititative test can be done to determine if the mushrooms contain amatoxin?
Wieland-Meixner test. The Meixner test has been shown to identify alpha-amanitin. This is a qualitative test and there have been false positive reactions caused by psilocin and other mushrooms.
Beuhler M et al. The Meixner test in the detection of alpha-amanitin and false-positive reactions caused by psilocin and 5-substituted tryptamines. Ann Emerg Med 2004 Aug;44(2):114-20. doi: 10.1016/j.annemergmed.2004.03.017.